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The effects of cold rolling reduction and annealing temperatures on the formation of cube texture and microstructural evolution have been investigated in Cu-44%Ni alloy. The results showed that the recrystallized cube texture is strengthened by either increasing the rolling reduction or increasing the annealing temperature. And the strong cube texture can be observed for the severely cold-rolled (> 90%) alloy after annealing at high temperature (> 900 °C). On the other hand, high-angle grain boundaries (HAGBs) and Annealing twin boundaries (Σ3 boundaries) decreases with the increase of rolling reduction. However, during isochronal annealing, the trend of Σ3 boundaries is consistent with that of HAGBs, which firstly increase during recrystallization process and then decrease with further increasing temperature. After annealing at 1100 °C for 1 h, the 99% cold-rolled Cu-44%Ni revealed that the fraction of the cube texture is 99.8% and the fractions of HAGBs and Σ3 boundaries are 2.5% and 1.3%, respectively.