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Mg-4Zn合金表面摻鍶羥基磷灰石涂層:物理化學(xué)性質(zhì)及體外細(xì)胞響應(yīng)
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湖南大學(xué)生物學(xué)院,湖南大學(xué)生物學(xué)院,中國科學(xué)院深圳先進(jìn)技術(shù)研究院,湖南大學(xué)生物學(xué)院,湖南大學(xué)生物學(xué)院,湖南大學(xué)生物學(xué)院

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國家自然科學(xué)基金青年基金項(xiàng)目,編號51604104;深圳市發(fā)改委項(xiàng)目,編號JCYJ20140417113430610


The Strontium-doped hydroxyapatite coatings deposited on Mg-4Zn alloy: Physical-chemical properties and in vitro cell response
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College of Biology,Hunan University, Changsha,China,College of Biology,Hunan University, Changsha,China,Shenzhen Institutes of Advanced Technology,Chinese Academy of Sciences,College of Biology,Hunan University, Changsha,China,College of Biology,Hunan University, Changsha,China,College of Biology,Hunan University, Changsha,China

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Project (No.51604104) supported by the Natural Science Foundation of China; Project (JCYJ20140417113430610) supported by the Development and Reform Commission of Shenzhen Municipality.

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    摘要:

    生物可降解鎂合金由于具有高比強(qiáng)度、低彈性模量和優(yōu)良生物相容性而受到廣泛關(guān)注。然而,其過高的腐蝕速率卻限制了其潛在的臨床應(yīng)用。因此,具有高生物相容性的羥基磷灰石(HA)涂層常被用于阻礙鎂基體和周圍生物環(huán)境的相互作用。采用電化學(xué)沉積法在Mg-4Zn合金表面制備了HA和摻鍶(Sr)HA涂層。利用掃描電子顯微鏡(SEM)、能譜(EDS)、透射電子顯微鏡(TEM)、三維激光掃描顯微鏡(3D LSM)和親水角監(jiān)測系統(tǒng)對材料表面性質(zhì)進(jìn)行表征。本研究還探討了材料動(dòng)態(tài)離子釋放、蛋白吸附、細(xì)胞吸附、增殖與成骨分化行為。結(jié)果顯示,HA中引入Sr導(dǎo)致了晶格畸變和結(jié)晶度下降。涂覆摻Sr HA的樣品中鎂離子釋放量比其他樣品更低,說明耐腐蝕性更好。摻Sr樣品表面蛋白吸附與初始細(xì)胞吸附的改善是由于其具有更高的表面粗糙度和親水性。Sr的引入并未顯著改變細(xì)胞的增殖,卻明顯提高了成骨分化效果。綜上所述,摻Sr HA涂層是一種非常有前景的鎂合金保護(hù)性生物相容涂層。

    Abstract:

    Biodegradable magnesium (Mg)-based biomaterials have draw extensively attention, due to the high strength-to-weight ratio, low elastic modulus and good biocompatibility. However, the high corrosion rate is still the major obstacle for the potential clinical applications. Therefore, the highly biocompatible hydroxyapatite (HA) coatings are usually introduced to restrain the interactions between Mg-based substrate and the body fluid environment. HA and strontium (Sr)-doped HA coatings were prepared on Mg-4Zn substrates by electrochemical deposition. The surface properties of the samples were characterized by scanning electron microscopy (SEM), energy dispersive spectroscopy (EDS), transmission electron microscopy (TEM), three-dimensional laser scanning microscopy (3D LSM) and a contact angle video system. The dynamic ion release, protein adsorption, cell adhesion, proliferation and differentiation behavior of the samples were also evaluated. The results reveal that the incorporation of Sr in the HA coatings leads to lattice distortion and decreased crystallinity. The lower amount of Mg ion release of the Sr-doped HA coated samples suggests a better corrosion resistance. The improved protein adsorption and initial adhesion of mesenchymal stem cells (MSCs) of the Sr-doped samples should be due to their higher surface roughness and wettability. The introduction of Sr leads to comparable cell proliferation behavior, but significantly improved osteogenic differentiation. It is concluded that the Sr-doped HA coatings are promising candidates for the protective biocompatible coating on Mg-based implants.

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引用本文

石偉,趙大鵬,尚鵬,聶和民,張遠(yuǎn),唐金成. Mg-4Zn合金表面摻鍶羥基磷灰石涂層:物理化學(xué)性質(zhì)及體外細(xì)胞響應(yīng)[J].稀有金屬材料與工程,2018,47(8):2371~2380.[SHI Wei, ZHAO Dapeng, SHANG Peng, NIE Hemin, ZHANG Yuan, TANG Jincheng. The Strontium-doped hydroxyapatite coatings deposited on Mg-4Zn alloy: Physical-chemical properties and in vitro cell response[J]. Rare Metal Materials and Engineering,2018,47(8):2371~2380.]
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  • 收稿日期:2018-06-30
  • 最后修改日期:2018-08-20
  • 錄用日期:2018-08-07
  • 在線發(fā)布日期: 2018-10-17
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