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Commercial pure titanium was selected to be a model material. Commercial pure titanium plates having different types of dislocation boundaries were prepared by multi-pass cold rolling. As-impacted titanium samples were obtained by the split Hopkinson pressure bar, and the evolution of dislocation boundary was characterized through transmission electron microscopy analysis. Through these, The high-speed deformation response of dislocation boundaries in commercial pure titanium was launched. It was demonstrated that the initial dislocation boundary became a major obstacle to dislocation slipping under high-speed compression at the strain rate of 1000/s. Plates distributed with geometrically necessary boundaries at the spacing of 0.5um can generate new dislocation boundaries intersected with the initial ones after high-speed deformation. When the spacing of geometrically necessary boundaries was about 0.3um, the dislocation groups formed among them. As the spacing of geometrically necessary boundaries reduced to 0.1um or below, the localized microstructure mode was bending of initial boundary and dislocation groups. There were only dislocation groups and sub-grain in the highly localized zone.