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純鈦材與大變形純鈦材微弧氧化-水熱合成復合陶瓷膜層的細胞相容性研究
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江蘇大學機械工程學院,常州大學,江蘇大學機械工程學院,江蘇大學實驗動物中心,江蘇大學實驗動物中心

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R318；TB333

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江蘇省高校自然基金重大項目(11KJA430004),江蘇省高校自然基金(12KJD460002)資助


Cytocompatibility of Composite Ceramic Coating on Titanium and Large Plastic Deformed Titanium by Micro-arc Oxidization-Hydrothermal Synthesis
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    摘要:

    采用微弧氧化-水熱法分別在純鈦材及大變形純鈦材表面制備了TiO2/HA復合陶瓷膜層，從細胞毒性試驗、細胞增殖實驗、細胞黏附實驗等方面評價膜層的細胞相容性。結(jié)果表明：純鈦材與大變形純鈦材微弧復合陶瓷膜層均無細胞毒性。與純鈦材微弧復合陶瓷膜層相比，大變形純鈦材微弧復合陶瓷膜層表面粗糙度更適宜，結(jié)晶形核的HA晶粒的形狀及Ca/P比更接近人骨HA，更能有效促進成骨細胞的黏附和鋪展，隨著培養(yǎng)時間的延長，成骨細胞雙層重疊生長結(jié)構(gòu)更為明顯。成骨細胞在大變形純鈦材微弧復合陶瓷膜層表面各時間點的吸光度值均更高，細胞相容性更好。

    Abstract:

    Micro-arc oxidation(MAO) and hydrothermal synthesis(HS) methods were used to prepare hydroxyapatite(HA)and anatase TiO2 composite ceramic coating on the surface of conventional titanium and large plastic deformed titanium. The cellular compatibility of modified coating was evaluated by cytotoxicity experiment,cell proliferation assay and adhesion behavior of osteoblasts. The results demonstrated that: All samples were found to be noncytotoxic. Compared with the composite ceramic coating of conventional titanium, needle-like HA crystallization, which was closer to the Ca/P ratio of natural human bone, appeared on the surface of composite ceramic coating on large plastic deformed titanium. The composite ceramic coating of large plastic deformed titanium showed smoother and more level surface optimal and could better promote adhesion and spreading of osteoblasts.Optical density of osteoblasts cultured on the composite ceramic coating of large plastic deformed titanium was also significantly higher, and the two-tier growing structure of osteoblasts was more obvious with the progression of cultural time, which implied optimal cytocompatibility.

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徐琳,丁建寧,許曉靜,何遠清,雷小春.純鈦材與大變形純鈦材微弧氧化-水熱合成復合陶瓷膜層的細胞相容性研究[J].稀有金屬材料與工程,2017,46(2):548~554.[Xu Lin, Ding Jianning, Xu Xiaojing, He Yuanqing, Lei Xiaochun. Cytocompatibility of Composite Ceramic Coating on Titanium and Large Plastic Deformed Titanium by Micro-arc Oxidization-Hydrothermal Synthesis[J]. Rare Metal Materials and Engineering,2017,46(2):548~554.]
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  • 收稿日期:2015-01-06
  • 最后修改日期:2015-07-29
  • 錄用日期:2015-10-13
  • 在線發(fā)布日期: 2017-04-10
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