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生物活性鈦體內(nèi)抑菌性能及其抑菌機(jī)理研究
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國家自然科學(xué)基金(31070848, 50961003, 30870615);“973”計(jì)劃項(xiàng)目(2012CB933603);四川省青年科學(xué)基金項(xiàng)目(09ZQ026-033);教育部博士點(diǎn)基金項(xiàng)目(20110181110064);四川大學(xué)口腔疾病研究國家重點(diǎn)實(shí)驗(yàn)室開放基金項(xiàng)目


Antibacterial Properties of Bioactive Ti in Vivo and Its Mechanism
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    摘要:

    為研究堿熱處理的鈦(AH-Ti)的體內(nèi)抑菌性能及其機(jī)理,以純鈦為對(duì)照,將其植入大鼠體內(nèi),并注入金黃色葡萄球菌液引起感染。通過測定髓過氧化酶活性評(píng)價(jià)感染程度,用酶聯(lián)免疫法測定白蛋白和纖維蛋白原的濃度。結(jié)果表明,AH-Ti的細(xì)菌感染程度較低,白蛋白和纖維蛋白原濃度也較低。本研究證實(shí)了AH-Ti在生理環(huán)境下具有一定的抑菌性能,其機(jī)理為AH-Ti表面選擇性吸附白蛋白而阻止了細(xì)菌粘附,從而減輕炎癥反應(yīng),進(jìn)而使纖維蛋白原的濃度也較低

    Abstract:

    In order to study the antibacterial properties of bioactive Ti and its mechanism, alkali-heat treated titanium (AH-Ti) was implanted in rat with Staphylococcus aureus suspension to make infection with pure titanium as contrast. The myeloperoxidase activity was detected for evaluating the rat’s inflammation. The concentrations of albumin and fibrinogen were measured by the Enzymemultiplied immunoassay technique. The results show the AH-Ti related inflammation is low along with low albumin and fibrinogen. It is proved AH-Ti has good antibacterial property in vivo. The mechanism for AH-Ti to prevent bacteria adhering to its surface is that its selectively absorbing albumin to its surface weakens the inflammation around the AH-Ti, and the fibrinogen concentration is lower in turn

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張德茂,鄒 文,張其翼,楊幫成.生物活性鈦體內(nèi)抑菌性能及其抑菌機(jī)理研究[J].稀有金屬材料與工程,2013,42(7):1483~1487.[Zhang Demao, Zou Wen, Zhang Qiyi, Yang Bangcheng. Antibacterial Properties of Bioactive Ti in Vivo and Its Mechanism[J]. Rare Metal Materials and Engineering,2013,42(7):1483~1487.]
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  • 收稿日期:2012-07-23
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