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Ru/C catalysts were prepared by supercritical CO2 deposition using RuCl3 as active precursor and activated carbon as support. The activity of Ru/C catalysts for hydrogenation of glucose were tested under 4.0 MPa and at 120 oC. The effects of the co-solvent category, co-solvent amount and supercritical CO2 pressure on catalytic activity were tested. The surface morphology, crystal form and Ru distribution of Ru/C catalysts were investigated by SEM, XRD and XPS. The results show that supercritical CO2 deposition technology can increase the activity of the Ru/C catalysts. In the experimental range, optimal activity of the catalysts can be obtained under the appropriate conditions of 2 ml methanol co-solvent and supercritical CO2 pressure 12.0 MPa. The highest reaction rate for hydrogenation of glucose exhibits 1.48 times higher than that of the catalyst prepared by the traditional method. Ru exists in the amorphous form in the catalysts with good dispersion on the activated carbon. Supercritical fluid deposition can enhance interaction between Ru and activated carbon.