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多孔鎂表面生物活性β-TCP涂層的制備及其細(xì)胞相容性研究
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中國(guó)科學(xué)院知識(shí)創(chuàng)新工程重要方向項(xiàng)目(KGCX2-YW-207)


Preparation and Cytocompatibility of Bioactive β-TCP Coatings on Porous Magnesium Scaffold Surface
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    摘要:

    采用化學(xué)沉積法在鎂支架表面制備生物活性的β-TCP(磷酸三鈣陶瓷)涂層，利用X射線衍射，掃描電子顯微鏡研究β-TCP涂層的相結(jié)構(gòu)和表面形貌。并對(duì)表面改性的鎂支架與類(lèi)成骨細(xì)胞UMR106的體外生物相容性進(jìn)行了詳細(xì)研究。結(jié)果表明，表面改性后的鎂支架浸提液細(xì)胞毒性為1級(jí)，即無(wú)細(xì)胞毒性；材料浸提液無(wú)細(xì)胞DNA毒性，對(duì)細(xì)胞周期無(wú)改變，也無(wú)異倍體細(xì)胞出現(xiàn)。實(shí)驗(yàn)結(jié)果證明，表面改性后的多孔鎂具有良好的細(xì)胞相容性，是一種很有前景的新型骨組織工程支架材料

    Abstract:

    A chemical deposition route was employed to prepare bioactive β-TCP coatings on porous magnesium scaffold surfaces. The phase identification and surface morphology of the specimens were studied by X-ray diffraction and scanning electron microscope, respectively. The cytocompatibility of treated porous magnesium scaffolds was studied in the present paper by using human osteoblast-like UMR106 cells. It was found that the cytotoxicity was in grade 1, indicating cytotoxicity free and without damage to DNA and no change to the cell cycle for the extracted liquid of treated magnesium. All the experimental results indicate that the treated porous magnesium has a good cytocompatibility and potential to be a new type of bone tissue engineering scaffolds

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耿 芳,譚麗麗,賀永蓮,楊敬玉,張炳春,楊 柯.多孔鎂表面生物活性β-TCP涂層的制備及其細(xì)胞相容性研究[J].稀有金屬材料與工程,2009,38(2):318~322.[Geng Fang, Tan Lili, He Yonglian, Yang Jingyu, Zhang Bingchun, Yang Ke. Preparation and Cytocompatibility of Bioactive β-TCP Coatings on Porous Magnesium Scaffold Surface[J]. Rare Metal Materials and Engineering,2009,38(2):318~322.]
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  • 收稿日期:2008-01-23
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