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可見光響應(yīng)摻氮TiO2的制備及對(duì)白血病HL60細(xì)胞體外殺傷效應(yīng)的研究
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O643.36 R730.53

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廣東省自然科學(xué)基金（031502）


Study on Preparation oF Visible Light-Activated Nitrogen-Doped TiO2 and Its Photodesdrucing Effect on Leukemic HL60 Cells in Vitro
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    摘要:

    采用硫酸鈦與氨水反應(yīng)制備摻氮TiO2。UV-Vis漫反射譜研究表明摻氮的TiO2在紫外區(qū)的吸收邊明顯紅移（約20nm），并且在400nm-530nm出現(xiàn)新的吸收區(qū)域，光吸收閾值從380nm擴(kuò)展到530nm。XPS分析表明，在TiO2摻雜的N為間隙態(tài)，而且還存在大量的氧空位。氧空位在導(dǎo)帶下形成的窄帶使得摻氮的TiO2在400nm～530nm出現(xiàn)新的吸收區(qū)域。用MTT法測(cè)量了摻氮TiO2殺傷白血病HL60細(xì)胞的活性。結(jié)果顯示，無(wú)論有無(wú)可見光照射，摻氮TiO2對(duì)白血病腫瘤細(xì)胞殺滅效果明顯好于未摻雜TiO2。

    Abstract:

    Nitrogen-doped TiO2 was prepared by Ti(SO4)2 solution reacting with NH3 aqueous solution. The optical absorption edge of nitrogen-doped TiO2 shifted 20 nm toward the infrared and also absorbs the visible light from 400 nm to 530 nm compared with undoped TiO2.Band gap of undoped TiO2 was 3.24 eV and nitrogen-doped TiO2 was 3.14 eV. X-ray photoelectron spectroscopy(XPS) measurements show that the nitrogen in N-doped TiO2 is in dissociative interstitial state. That the new absorption band at 400 nm ~530 nm is induced by oxygen vacancies in N-doped TiO2, which exhibits a narrow band close to the conduction band. The MTT assay was used for detecting the activity of N-doped TiO2 on the growth of leukemic HL60 in vitro. The results indicate that N-doped TiO2 can improve the efficiency of destructing leukemic HL60 cells no matter there is or not visible-light irradiation.

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熊予瑩 費(fèi)賢翔 熊建文 張鎮(zhèn)西.可見光響應(yīng)摻氮TiO2的制備及對(duì)白血病HL60細(xì)胞體外殺傷效應(yīng)的研究[J].稀有金屬材料與工程,2006,35(11):1735~1739.[Xiong Yuying, Fei Xianxiang, Xiong Jianwen, Zhang Zhenxi. Study on Preparation oF Visible Light-Activated Nitrogen-Doped TiO2 and Its Photodesdrucing Effect on Leukemic HL60 Cells in Vitro[J]. Rare Metal Materials and Engineering,2006,35(11):1735~1739.]
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  • 收稿日期:2006-06-26
  • 最后修改日期:2006-06-26
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