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鈦表面處理后表面能分析及對細(xì)胞附著的影響
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TG146.4

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中法先進(jìn)研究計(jì)劃合作項(xiàng)目(PRA BT00-07)，西安市工業(yè)科技攻關(guān)計(jì)劃項(xiàng)目(GG200356)


Surface Energy Analysis of Treated Titanium and Effects on Cell Adhesion
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    摘要:

    采用微弧氧化（MAO）及水熱合成（MAO-HS）法對純鈦表面進(jìn)行處理，然后對其表面能進(jìn)行分析，并觀察成骨細(xì)胞在其表面的附著動力學(xué)變化及骨架蛋白表達(dá)。結(jié)果顯示MAO表面能（50.6mj/m2）。細(xì)胞在MAO-HS表面的附著率明顯高于MAO及Ti表面。細(xì)胞骨架蛋白在3種材料表面均表達(dá)良好，但彼此之間存在差異。因此鈦有面采用微弧氧化及水熱合成法處理后表面形貌及表面能發(fā)生改變，這種改變影響了細(xì)胞在其表面的生物為行為。

    Abstract:

    In this study the micro-arc oxidized (MAO) and hydrothermally synthesized (MAO-HS) methods were used to modify the TiO2 layer of titanium. The surface characters and the effects on cell behavior, which included cell adhesion kinetics and cytoskeletal organizations were need to evaluate. The surface energy was examined by physicochemical methods. Biological assays were performed with the mouse osteoblastic cell line MC3T3-E1, cells were inoculated on the treated surface of the materials. The surface energy of MAO (50.6 mJ/m2) and MAO-HS (58.5 mJ/m2) was higher than titanium (39.6 mJ/m2 ). A significantly higher cell adhesion rate was observed on the MAO-HS surface than on the MAO and titanium surface. The cytoskeletal organizations were analyzed by actin and vinculin staining on all the samples after being cultured 2 hours. Modification of TiO2 layer on titanium surface has an influence on the surface characters and the cell behavior on the materials.

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張玉梅 趙銥民 黃平 憨勇 PBataillon-linez HFHildebrand MTraisnel.鈦表面處理后表面能分析及對細(xì)胞附著的影響[J].稀有金屬材料與工程,2004,33(5):518~521.[Zhang Yumei, Zhao Yimin, Huang Ping, Han Yong, P. Bataillon-linez, H. F. Hildebrand, M. Traisnel. Surface Energy Analysis of Treated Titanium and Effects on Cell Adhesion[J]. Rare Metal Materials and Engineering,2004,33(5):518~521.]
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  • 最后修改日期:2003-06-25
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