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骨髓成骨細(xì)胞在堿熱處理的磷灰石涂層鈦表面的分化增殖研究
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Osteoblast Differentiation of Bone Marrow Cells Cultured on Apatite-Coated Titanium by Alkali-Heat Treatment
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    摘要:

    為了研究骨髓基質(zhì)細(xì)胞轉(zhuǎn)化的成骨細(xì)胞在堿熱處理的涂層磷灰石鈦表面的分化增殖，將鈦片經(jīng)過(guò)堿熱處理后，浸泡于與類(lèi)似體液的環(huán)境進(jìn)行表面磷灰石涂層。應(yīng)用骨髓成骨細(xì)胞作為實(shí)驗(yàn)工具，觀察體外細(xì)胞在處理的涂層鈦片上的生長(zhǎng)，堿性磷酸酶以及降鈣素的分泌。在早期，有磷灰石涂層的鈦片成骨細(xì)胞黏附高于對(duì)照組；在晚期，兩組之間沒(méi)有明顯差異；成骨細(xì)胞分化的標(biāo)志——堿性磷酸酶活性和降鈣素的表達(dá)，磷灰石涂層組都明顯高于對(duì)照組。研究表明：堿熱處理的磷灰石涂層鈦能夠促進(jìn)成骨細(xì)胞黏附，有利于細(xì)胞向成骨分化。

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    Osteoblast differentiation of rabbit bone marrow cells on apatite-coated titanium by alkali-heat treatment was investigated in present paper. Titanium was subjected to alkali-heat treatment and soaked in acellular simulated body fluid to prepare surface apatite layer. Cell behavior and alkaline phosphatase activity and osteocalcin on apatite-coated titanium were compared with those on commercially pure titanium in vitro. The results show that initial cell adhesion on apatite-coated titanium is higher than that on pure titanium while cell growth rates at a late stage are not significantly different for both groups. Early and later osteoblast differentiation marks, alkaline phosphatase activity and osteocalcin, on apatite-coated titanium are higher than those on pure titanium. Apatite-coated titanium by alkali-heat treatment can increase osteoblast adhesion and displays a higher differentiation capacity for osteoblasts differentiation than that for commercially pure titanium.

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彭磊 萬(wàn)明習(xí) 梁芳慧 孫崢 常琪 王臻.骨髓成骨細(xì)胞在堿熱處理的磷灰石涂層鈦表面的分化增殖研究[J].稀有金屬材料與工程,2004,33(10):1018~1022.[Peng Lei, Wan Mingxi, Liang Fanghui, Sun Zheng, Chang Qi, Wang Zhen,. Osteoblast Differentiation of Bone Marrow Cells Cultured on Apatite-Coated Titanium by Alkali-Heat Treatment[J]. Rare Metal Materials and Engineering,2004,33(10):1018~1022.]
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