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In this study, grazing incidence X-ray diffraction（GIXRD）, elastic recoil detection（ERD）, thermal desorption spectroscopy（TDS） and scanning electron microscope（SEM） were used to analyze helium-containing Zr-Co and Zr-Ti-Co films prepared by direct current magnetron sputtering, and the sample phase, the depth and concentration distribution of helium，helium thermal desorption spectrometry and surface morphology before and after thermal desorption were obtained. The helium thermal desorption experiment shows that Ti doping is beneficial to inhibit the migration, accumulation and evolution of helium in ZrCo tritium storage alloy and increase the release temperature of helium in corresponding trapping sites. After helium thermal desorption, the grain boundary density of Zr0.8Ti0.2Co films is lower than that of ZrCo films, which reduces the helium bubble migration channel and delays the helium bubble migration and merger trend at high temperature, indicating that Ti-doped can improve the helium retention property of ZrCo alloy and inhibit the helium release at high temperature.